| Abstract | Detection of Genetic Diversity in Campylobacter jejuni Isolated from a Commercial Turkey Flock Using flaA Typing, MLST Analysis and Microarray Assay
Hosny El-Adawy, Helmut Hotzel1, Herbert Tomaso1, Heinrich Neubauer1, Eduardo N. Taboada,
Ralf Ehricht, Hafez M. Hafez
Friedrich-Loeffler-Institut, Institute of Bacterial Infections and Zoonoses, Jena, Germany, 2 Institute for Poultry Diseases, Free University Berlin, Berlin, Germany,
Department of Poultry Diseases, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh, Egypt, 4 Laboratory for Foodborne Zoonoses, Public Health
Agency of Canada, Lethbridge, Canada, 5 Alere Technologies GmbH, Jena, Germany
ABSTRACT
Campylobacter is genetically highly diverse and undergoes frequent intraspecific recombination. Turkeys have been identified as an important reservoir for Campylobacter jejuni which is of public health significance. The assessment of the genetic diversity among Campylobacter population is critical for our understanding of the epidemiology of this bacterium. The genetic profiles were different according to the molecular typing methods used. The performance of established flaA genotyping, multilocus sequencing typing (MLST) and DNA microarray assay based on the ArrayTubeTM technology was evaluated using 14 Campylobacter jejuni isolated from a commercial turkey flock. The flaA typing was performed using PCRRFLP with restriction enzymes Sau3AI, AluI, a ‘composite’ flaA analysis of AluI and Sau3AI and DdeI. The 14 isolates were differentiated into 3, 5, 7 and 9 genotypes, respectively. Entire flaA gene and short variable region (SVR) sequences were analysed. Sequencing of the entire flaA provided 11 different genotypes. flaA-SVR sequence analysis detected 8 flaA alleles and 4 flaA peptides. One new flaA allele type (528) was identified. MLST analysis represented 10 different sequence types (STs) and 5 clonal complexes (CCs). The microarray assay recognised 14 different genotypes. The discriminatory indices were 0.560, 0.802, 0.857, and 0.912 for flaA-RFLP depending on the used enzymes, 0.890 for flaA-SVR, 0.967 for entire flaA sequencing, 0.945 for MLST and 1.00 for the DNA microarray assay. The flaA gene was genetically stable over 20 passages on blood agar. In conclusion, the different typing tools demonstrated a high level of genetic heterogeneity of Campylobacter jejuni in a turkey flock, indicating that a single flock can be infected by multiple genotypes within one rearing cycle. DNA microarray-based assays had the highest discriminatory power when compared with other genotyping tools. |