| Abstract | PRIMARY ISOLATION AND CHARACTERIZATION OF SPRING VIREMIA OF CARP VIRUS (SVCV) FROM CULTURED Fish IN KAFR EL-SHIKH GOVERNORATE
M.S.M. Gado ; Talaat T. Saad and Amira Alaa El-Dein
Department of Fish Diseases and hygiene, Faculty of Veterinary Medicine, KAFR EL-SHIKH University, Egypt
Department of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Alexandria University,Egypt
ABSTRACT
In the present study, Spring viremia of carp virus (SVCV ) was identified from cultured common carp (Cyprinus carpio), silver carp (Hypophthalmixis molitrix) and O. niloticus during disease out-break in a private fish farm in Egypt. The electron microscope was used to describe the viruse and determine their shape and form. Specific immunohistochemistery test was carried out using monoclonal antibody of SVCV and antimouse immunoglobulin to verify the presence of the SVCV. Histopathological investigation of the infected fishes was also carried out. The results revealed the presence of bullet-shape Rhabdovirus. The specificity of the isolated SVCV to antibody was proved. The clinical signs of infected fish included skin darkening, tail and fin rot, ulceration, hemorrhage on the abdomen, ascites and redness of isthmus and head region. Postmortem lesions were congestion of all internal organs, distended gall bladder, hemorrhagic gas bladder, exophthalmia and swelling of the anal opening. Histopathological examination of the infected organs by the described virus revealed degenerative changes and focal necrosis in the involved cells of the internal organs. Polymerase chain reaction is good prognosis of the virus presence. The present results indicated that the virus infection was likely the cause the infection and was responsible for the mortalities and lesions during the outbreak. This result may be the first report on spring viremia among cultured fishes in Egypt but it needs further studies for specification and characterization of the virus. |