Development of a voltammetric procedure for assay of the antihistamine drug hydroxyzine at a glassy carbon electrode: Quantification andpharmacokinetic studies

Abstract

An electrochemical study of hydroxyzine at a glassy carbon electrode was carried out in the Britton–Robinson universal buffer of pH 2–11. Hydroxyzinewas oxidized in a single two-electron irreversible process controlled mainly by adsorption.Asimple, sensitive and time-saving squarewave adsorptive anodic stripping voltammetric procedure has been developed for determination of hydroxyzine in its commercial tablets and human serum without prior extraction. The optimized procedural conditions were: frequency = 120 Hz, scan increment = 10mV, pulse-amplitude = 25mV, accumulation potential =?0.3V, accumulation time = 90–300 s and a Britton–Robinson universal buffer of pH 4 as a supporting electrolyte. Mean recoveries of 100.5±0.71 and 98.6±1.12% (n = 5) were achieved for assay of hydroxyzine in Atarax® 10 and 25 mg dosage forms, respectively. Limit of detection of 1.5×10?8 mol L?1 (5.624 ngmL?1) and limit of quantitation of 5.0×10?8 mol L?1 (18.746 ngmL?1) were achieved in human serum with a mean recovery of 98.4±1.22%, without prior extraction of the drug. Moreover, the described procedure was applied for evaluating the pharmacokinetic parameters of hydroxyzine in plasma of two healthy volunteers after administration of a single oral dose (Atarax®—25 mg). © 2007 Elsevier B.V. All rights reserved.

 

 

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